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caspase 3  (Beyotime)


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    Structured Review

    Beyotime caspase 3
    Caspase 3, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+3+inhibitor+ac+devd+cho/Caspase+3+Inhibitor+Ac-DEVD-CHO/pm42848511-370-19-21
    Average 99 stars, based on 82 article reviews
    caspase 3 - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    CCK-8 Assay:

    Article Title: Schisandrin B induces HepG2 cells pyroptosis by activating NK cells mediated anti-tumor immunity.
    Article Snippet: Pyroptosis, an inflammatory programmed cell death, has been suggested as a novel molecular mechanism for the treatment of hepatocellular carcinoma (HCC) with chemotherapeutic agents.. Recent studies showed that natural killer (NK) cells could inhibit apoptosis and regulate the progression of pyroptosis in tumor cells.. Schisandrin B (Sch B), a lignan isolated from Schisandrae chinensis (Turcz.)

    LDH Cytotoxicity Assay:

    Article Title: Schisandrin B induces HepG2 cells pyroptosis by activating NK cells mediated anti-tumor immunity.
    Article Snippet: Pyroptosis, an inflammatory programmed cell death, has been suggested as a novel molecular mechanism for the treatment of hepatocellular carcinoma (HCC) with chemotherapeutic agents.. Recent studies showed that natural killer (NK) cells could inhibit apoptosis and regulate the progression of pyroptosis in tumor cells.. Schisandrin B (Sch B), a lignan isolated from Schisandrae chinensis (Turcz.)

    Article Title: Foot-and-Mouth Disease Virus Induces Porcine Gasdermin E-Mediated Pyroptosis through the Protease Activity of 3C pro .
    Article Snippet: Foot-and-mouth disease (FMD) is an acute, highly contagious disease of cloven-hoofed animals caused by FMD virus (FMDV).. Currently, the molecular pathogenesis of FMDV infection remains poorly understood.. Here, we demonstrated that FMDV infection induced gasdermin E (GSDME)-mediated pyroptosis independent of caspase-3 activity.

    Bicinchoninic Acid Protein Assay:

    Article Title: Schisandrin B induces HepG2 cells pyroptosis by activating NK cells mediated anti-tumor immunity.
    Article Snippet: Pyroptosis, an inflammatory programmed cell death, has been suggested as a novel molecular mechanism for the treatment of hepatocellular carcinoma (HCC) with chemotherapeutic agents.. Recent studies showed that natural killer (NK) cells could inhibit apoptosis and regulate the progression of pyroptosis in tumor cells.. Schisandrin B (Sch B), a lignan isolated from Schisandrae chinensis (Turcz.)

    Injection:

    Article Title: Caspase-3 interactions with calpain and cathepsin L: Implications for protein stability and quality in fish fillets during postmortem storage
    Article Snippet: The degradation of structural proteins during fish postmortem storage is a critical factor affecting meat quality, leading to economic losses in the seafood industry.. The complex interplay between caspase-3 and structural protein stability during fish postmortem storage remains largely unexplored.. By treating grass carp fillets with a caspase-3 inhibitor, we establish a model for inhibiting apoptosis, delineating the role of caspase-3 in protein degradation.

    other:

    Article Title: Baicalin inhibits influenza A (H1N1)-induced pyroptosis of lung alveolar epithelial cells via caspase-3/GSDME pathway.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: No.81830101 Abstract Baicalin (7‐D‐glucuronic acid‐5, 6‐dihydroxyflavone) derived from the root of Scutellaria baicalensis used as Traditional Chinese Medicine (TCM) has been revealed to exert potential antiviral activity via various pathways, while the molecular mechanisms have not been fully understood.. Pyroptosis, an inflammatory form of programmed cell death (PCD), is reported to play a crucial role in host cell fate during viral infection.. In this study, transcriptome analysis of mice lung tissue reveals that baicalin reverses the alterations of the mRNA levels of PCD‐associated genes upon H1N1 challenge, with a concomitant decrease in the population of H1N1‐induced propidium iodide (PI) and Annexin V cells.

    Article Title: Isorhamnetin Suppresses Human Gastric Cancer Cell Proliferation through Mitochondria-Dependent Apoptosis.
    Article Snippet: Caspase-3 inhibitor Ac-DEVD-CHO (C1206), Hoechst 33258 (C1017), and Mito-Tracker Red CMXRos (C1049B) were purchased from Beyotime (Nantong, China).

    Article Title: Apoptotic caspase inhibits innate immune signaling by cleaving NF-κBs in both Mammals and Flies
    Article Snippet: The HEK293 cells stably expressing TLR3 (293-TLR3) were generously provided by Prof. Bo Zhong (Wuhan University, Wuhan, China).

    Article Title: Mammalian orthoreovirus capsid protein σ3 antagonizes RLR-mediated antiviral responses by degrading MAVS
    Article Snippet: Human IFN-β ELISA Kit (Shanghai Enzyme-Linked Biotechnology, Shanghai, China) was used to detect the expression of IFN-β in the cell supernatant according to the manufacturer’s instructions.

    MTT Assay:

    Article Title: Dual Nanozyme-Driven PtSn Bimetallic Nanoclusters for Metal-Enhanced Tumor Photothermal and Catalytic Therapy.
    Article Snippet: Platinum (II) acetylacetonate (Pt(acac)2, 48% Pt basis), tin (II) chloride dihydrate (SnCl2·2H2O, 98.0%), tetrabutylammonium borohydride (TBAB, 95%), oleylamine (OAm, 80−90%), 1-octadecene (ODE, 90%), chloroform, SH-PEG-NH2, acetone, ophenylenediamine (OPD), 3,3′,5,5′-tetramethyl-benzidine (TMB), 5,5-dimethyl-1-pyrrolineN-oxide (DMPO), and ethanol were purchased from Sigma-Aldrich (Shanghai, China). .. Methylthiazolyldiphenyl-tetrazolium bromide (MTT), 4′,6-diamidino-2-phenylindole (DAPI), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), Calcein-AM and propidium iodide (PI), JC-1 staining kit, ActinGreen, ActinRed, caspase-3 inhibitor Ac-DEVD-CHO, and Annexin VFITC/PI apoptosis detection kit were purchased from Beyotime Inst. ..

    Staining:

    Article Title: Dual Nanozyme-Driven PtSn Bimetallic Nanoclusters for Metal-Enhanced Tumor Photothermal and Catalytic Therapy.
    Article Snippet: Platinum (II) acetylacetonate (Pt(acac)2, 48% Pt basis), tin (II) chloride dihydrate (SnCl2·2H2O, 98.0%), tetrabutylammonium borohydride (TBAB, 95%), oleylamine (OAm, 80−90%), 1-octadecene (ODE, 90%), chloroform, SH-PEG-NH2, acetone, ophenylenediamine (OPD), 3,3′,5,5′-tetramethyl-benzidine (TMB), 5,5-dimethyl-1-pyrrolineN-oxide (DMPO), and ethanol were purchased from Sigma-Aldrich (Shanghai, China). .. Methylthiazolyldiphenyl-tetrazolium bromide (MTT), 4′,6-diamidino-2-phenylindole (DAPI), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), Calcein-AM and propidium iodide (PI), JC-1 staining kit, ActinGreen, ActinRed, caspase-3 inhibitor Ac-DEVD-CHO, and Annexin VFITC/PI apoptosis detection kit were purchased from Beyotime Inst. ..



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    Effects of BHJ on the expression and activity of apototic-related proteins in HepG2 cells. A Western blot analysis of apoptotic-associated protein levels in HepG2 cells; B , C Effect of BHJ on caspase 9, caspase 3 enzyme activities in HepG2 cells; D Inhibition of BHJ-induced apoptosis by caspase 9 and caspase 3 inhibitors in HepG2 cells. The values are expressed as means ± SD of triplicates tests. * P < 0.05 comparison between the absence and presence of inhibitor in the treatment group

    Journal: BMC Complementary Medicine and Therapies

    Article Title: Antioxidant activity and cytotoxic effects of Benincasa hispida juice on HepG2 cells

    doi: 10.1186/s12906-026-05475-3

    Figure Lengend Snippet: Effects of BHJ on the expression and activity of apototic-related proteins in HepG2 cells. A Western blot analysis of apoptotic-associated protein levels in HepG2 cells; B , C Effect of BHJ on caspase 9, caspase 3 enzyme activities in HepG2 cells; D Inhibition of BHJ-induced apoptosis by caspase 9 and caspase 3 inhibitors in HepG2 cells. The values are expressed as means ± SD of triplicates tests. * P < 0.05 comparison between the absence and presence of inhibitor in the treatment group

    Article Snippet: To test the activity of caspase 3 and caspase 9, 10 μM of Z-LEHD-FMK (BioVision, CA, USA) or 20 μM Ac-DEVD-CHO (Beyotime, Haimen, China) were added and incubated for 2 h prior to addition of BHJ for 48 h, then cell viability was detected by alamar blue assay.

    Techniques: Expressing, Activity Assay, Western Blot, Analysis, Inhibition, Comparison